#Black light aquarium plus#

The most common way to measure these endpoints is by using colorimetric or fluorometric assays which involve the addition of an indicator dye. It is important to systematically combine multiple endpoints, such as membrane integrity and cell death mechanism, to gain insight into the potential pathways involved in cellular toxicity. These endpoints can include membrane integrity, mitochondrial function, proliferation, and apoptosis versus necrosis. This work presents the approach used to customize dye pairs for membrane integrity-based cell viability assays considering the species- and genotype-specific autofluorescence of scleractinian corals, namely: endogenous fluorescence characterization followed by the selection of dyes that do not overlap with endogenous signals.Ĭytotoxicity, the potential to cause cell death, is measured via different endpoints 1. damicornis cells exposed for 24 h to Triton-X100, insulin or titanium dioxide (TiO 2) NPs, respectively, at concentrations ranging from 0.5 to 100 µg/mL, revealed a LC50 of 0.46 µg/mL for Triton-X100, 6.21 µg/mL for TiO 2 NPs and 33.9 µg/mL for insulin.

#Black light aquarium plus#

Our results show that this dye pair can be distinctly measured in the presence of fluorescent proteins plus chlorophyll. The cytotoxicity of different representative molecules, namely small organic molecules, proteins and nanoparticles (NP), was measured after 24 h of exposure using the fluorescent dye pair Hoechst 33342 and SYTOX orange. Using Pocillopora damicornis as a model, we characterized the autofluorescence and tested different fluorescent dye pair combinations to identify alternative viability indicators.

The application of established cell viability assays such as the commonly used trypan blue staining method to coral cells is not straightforward due to different culture parameters and different cellular features specific to mammalian cells compared to marine invertebrates.